商品编号


T1020S-250 preps



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Nucleic Acid Purification




250 preps







商品信息

Description:
?
The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to lo
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ng on the column, saving you a step. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations.Monarch? Nucleic Acid Purification Kits – Designed with sustain
ABI
lity in mind, Monarch kits use significantly less plastic and respons
IBL
y-sourced, recyclable packaging
View our videos on protocols, tips, and recycling Monarch.
Monarch DNA Cleanup Column Design
Monarch columns are designed for performance
Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.
Advantages:
Elute in as little as 6 μl
Prevent buffer retention and salt carry-over with optimized column design
Save time with fast, user-friendly protocols
No need to monitor pH or add isopropanol
Buffers and columns available separately
Significantly less plastic used when compared with other kits
Respons
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y-sourced and recyclable packaging
Specifications
DNA Sample Type:
double-stranded DNA from agarose gels
Binding Capacity:
up to 5 μg
DNA Size Range:
~50 bp to 25 kb
Typical Recovery:
DNA (50 bp to 10 kb): 70–90%
DNA (11–23 kb): 50–70%
Elution Volume:
≥ 6 μl
Purity:
A
260/280
> 1.8
Protocol Time:
10 minutes of spin and incubation time
Compat
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e Downstream
Applications:
ligation, restriction digestion, labeling and other enzymatic
manipulations, library construction and DNA sequencing.
Monarch DNA Gel Extraction Kit Protocol?
DNA purified from the Monarch DNA Gel Extraction Kit is recovered with similar efficiency and purity as the le
ADI
ng supplier, but is more highly concentrated, facilitating its use in downstream applications
One microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and purity data determined by
Nanodrop
? re
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ngs, as well as recovery calculations based on the eluted DNA concentration and recovered volume.?
Monarch DNA Gel Extraction Kit reproduc
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y recovers DNA over a broad range of molecular weights.?
A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved on a new gel with the remainder of the original mixture for comparison.?
DNA purified from agarose gels using the Monarch DNA Gel Extraction Kit can be reproduc
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y isolated and ligated.
Two micrograms of a 3 kb fragment with compat
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e ends was resolved on a 1% agarose gel, excised, and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total) was ligated using the Blunt/TA Ligase Master Mix (
NEB
#M0367). Representative samples from 5 replicates were resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder (
NEB
#N3232).
Learn More About Monarch
NEB
Monarch.com
Optimize your results with our unique column design
Enhance your DNA purification experience
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Kit Components
The following reagents are supplied with this product:
Store at (°C)
Concentration
Monarch? Gel Dissolving Buffer
25
1X
Monarch? DNA Wash Buffer
25
5X
Monarch? DNA Elution Buffer
25
1X
Monarch? DNA Cleanup Columns (5 μg)
25
Notes:
The kit should be stored at room temperature. Always keep buffer bottles tightly closed and keep columns sealed in the enclosed zip-lock bag. For information regarding the composition of buffers, please consult the Safety Data Sheets. Proper laboratory safety practices should be employed, including the use of lab coats, gloves and eye protection.