商品编号


MA-0101



品牌


Signosis



公司


Signosis



公司分类


miRNA Research




1 Ea


商品信息

Description:
MicroRNAs (miRNAs) are small noncoding RNAs that control gene expression at the posttranscriptional level through selectively binding to complementary messenger RNA sequences.? Approximately 30% of mammalian genes are regulated by these small RNA molecules, which?affect a variety of?
BIOLOG
ical functions, including development, cell differentiation, proliferation, apoptosis, and maintenance of stemness and imprinting.?
Northern blotting has been the most commonly used method for analyzing individual miRNAs, although recently a number of new approaches have been developed,?such as?the related real-time PCR and invader assays.?Low sensitivity and poor throughput are the main issues of Northern blot analysis.?To resolve this problem, ?
Signosis
has developed the?miRNA plate assay kits. The kits are highly sensitive, and the biochemical conversion of miRNA molecules into
CDN
As is not needed. The procedure of the assay is as simple as incubation and wash.
Principle:
In the proprietary miRNA plate assay, one miRNA molecule is flanked by a capture oligo and a biotinated detection oligo through two bridges oligos. One of the bridge oligos is partially hybridized with the miRNA molecule and the capture oligo and another with the miRNA and the detection oligo. The hybrid is immobilized onto plate through hybridization with an immobilized oligo and detected by a streptavidin-HRP conjugate and chemiluminecscent substrate. This hybrid structure is sensitive to the sequence of the miRNA molecule. One nucleotide difference will prevent the formation of the hybrid and therefore miRNA isoform can be differentiated, which normally is hard to tackle with Northern blot. In addition, the sensitivity of the assay is higher than miRNA Northern blot assay.

Data:
miRNA plate assay. Both miR-133 and miR-1 are preferentially expressed in cardiac and skeletal muscles and play essential roles in differentiation, proliferation and apoptosis of cardiac cells. Dysregulated miRNA expression has been correlated to diseased hearts in human patients and therefore, they are with great potentials as diagnostic
Marker
s and therapeutic targets for cardiovascular disease. (A) Schematice diagram of miRNA plate assay. (B) Expression of miR-133 and miR-1 was analyzed with 5ug of total RNA prepared from human sckeletal muscle, heart, and liver through Northern blot (top) and miRNA plat assay (bottom). (C) Detection of miR-133 expression was compared with Northern blot (top) and miRNA plate assay (bottom).
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