商品编号


AP-0009



品牌


Signosis



公司


Signosis



公司分类


miRNA Research




1 Ea


商品信息

Description:
Signosis
' Human miRNA array IV allows you to profile 100 miRNAs.
Applicable Grid:
List of Applicable miRNAs




miR-138

miR-139

miR-105

miR-130a

miR-135a

miR-147

miR-152

miR-154-3p

miR-184

miR-187



miR-189

miR-211

miR-212

miR-217

miR-22

miR-220

miR-299-3p

miR-299-5p

miR-301

miR-302a-3p



miR-302a-5p

miR-302b-3p

miR-302b-5p

miR-302c-3p

miR-302c-5p

miR-302d

miR-30d

miR-30e-3p

miR-30e-5p

miR-32



miR-320

miR-323

miR-324-3p

miR-324-5p

miR-325

miR-326

miR-328

miR-329

miR-33

miR-330



miR-331

miR-335

miR-337

miR-338

miR-339

miR-33b

miR-340

miR-345

miR-346

miR-34b



miR-34c

miR-361

miR-362

miR-363-3p

miR-363-5p

miR-365

miR-367

miR-369-3p

miR-369-5p

miR-370



miR-371

miR-373-5p

miR-374

miR-376a-3p

miR-376a-5p

miR-376b

miR-377

miR-378

miR-379

miR-380-3p



miR-380-5p

miR-381

miR-382

miR-383

miR-384

miR-409-5p

miR-410

miR-411

miR-412

miR-422a



miR-422b

miR-423

miR-424

miR-425

miR-425-5p

miR-429

miR-432-5p

miR-432-3p

miR-433

miR-448



miR-449

miR-449b

miR-450

miR-451

miR-452-5p

miR-452-3p

miR-453

miR-455

miR-483

U6

Principle:
miRNA is different from large messenger RNA in three aspects; (1) miRNAs are small size molecules with quite a big difference in abundance, (2) mature miRNAs co-exist with their precursor pre-miRNA and pri-miRNA, which only differ in length, and (3) many miRNAs are very closely related in sequences, such as isoforms, only differing by one or a few nucleotides. Therefore, the conventional mircoarray technologies cannot directly be applied to analyzing these molecules. A number of miRNA microarray products are commercially available, but they are either tedious in requiring pre-isolation of microRNA, lack the discriminative power to differentiate between isoforms, or are not sensitive enough to monitor low abundant miRNAs.
In our array assay, each miRNA molecule is targeted by two oligos that hybridize with the target miRNA to form a RNA/DNA duplex. When the sequences are perfectly matched, the oligos are aligned with the miRNA and the joint can be ligated by DNA ligase (figure 1). A single nucleotide difference among miRNAs will block either the hybridization or the ligation; Thus, miRNA isoforms can be differentiated. Due to the small size of miRNA, the hybrid might not be stable; Therefore, we introduce the stacking sequences. By extending these two oligos along with their complementary oligos, the st
ABI
lity is increased. Once the pair of oligos is ligated, the ligated molecules are subjected to linear amplification via T7 transcription into RNA in the presence of biotin-UTP, which are used as probes for array hybridization. To differentiate each isoform, we assigned unique tag sequences to the ligation oligos, so that single nucleotide differences are converted into unique tag sequences. In this way, each isoform can be easily distinguished by array hybridization.
The assay procedure includes three steps: (1) mix the miRNA with provided oligos to form miRNA/oligo hybrids; (2) select the hybrids and remove free oligos, and ligate miRNA-directed pairing of oligos to become a single DNA; and (3) amplify the ligated DNA with T7 transcription.

Data:

Human miRNA array IV analysis of miRNA expression in human liver and brain tissue.