商品编号


EXOFLOW96A-Tetra



品牌


SBI



公司


System Biosciences（SBI）



公司分类


exosome isolation








商品信息

.img-style{heigth:450px;width:450px;}
Overview:
Streamline affinity-based exosome immunopurification

With magnetic beads already pre-coupled to antibodies to the tetraspanin proteins CD63, CD81, and CD9, and delivered in a 96-well format,
SBI
’s Exo-Flow96 Tetra IP Kit simplifies high-throughput, antibody-based exosome isolation. Our magnetic bead-coupled anti-CD9 antibodies are extensively validated, and the high-quality Exo-Flow IP kit components ensure reliable, reproduc
IBL
e affinity-based exosome purification directly from serum or plasma. Exosomes can also be purified from other biofluids such as media, urine, and CSF, but must first be concentrated using either ExoQuick-TC
?
or ultracentrifugation.



Selection of well-validated antibodies for reliable and reproduc
IBL
e purification

Large-sized magnetic beads increase the efficiency of exosome capture

Available in 32- and 96-well formats for high-throughput, affinity-based exosome isolation


Even better, with our larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, enabling capture of billions of exosomes from one sample.


Each IP kit comes with all the necessary reagents for immunopurification—capture antibody pre-coupled to magnetic beads in 96-well clear plates (as 8-well strips) with covers, wash buffer, Exosome Elution Buffer, and clearing reagent (to remove Exosome Elution Buffer). A 96-well plate magnet, the Exo-FlowMag96, can be purchased separately (Cat.# EXOFLOWMAG-1).

Different formats for different needs

To facilitate a range of studies,
SBI
built the Exo-Flow IP system to be highly modular. We offer a range of magnetic bead-coupled antibodies in 32- and 96-well formats.




Cat.#

Kit





EXOFLOW32A-CD63

Exo-Flow32 CD63 IP kit



EXOFLOW32A-CD81

Exo-Flow32 CD81 IP kit



EXOFLOW32A-CD9

Exo-Flow32 CD9 IP kit



EXOFLOW32A-Tetra

Exo-Flow32 Tetra IP kit (CD9, CD63, CD81)



EXOFLOW96A-CD63

Exo-Flow96 CD63 IP kit



EXOFLOW96A-CD81

Exo-Flow96 CD81 IP kit



EXOFLOW96A-CD9

Exo-Flow96 CD9 IP kit



EXOFLOW96A-Tetra

Exo-Flow96 Tetra IP kit (CD9, CD63, CD81)




Supporting Data:

See isolation data using Exo-FLOW IP Kits

For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.



Figure 1. CD63 and CD9, two exosome
Marker
s, are re
ADI
ly detected in samples purified using Exo-Flow Kits.
Approximately 1 ?g of protein was loaded per well on a 4–20% gr
ADI
ent protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein
Marker
s.


Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes
.


Citations:
Protocol, IIEI. (2017) List of Components.
Product
. ;. Link: Product
Etzerodt, A, et al. (2017) Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of ‘soluble CD163’ in plasma.
Sci Rep
. 2017 Jan 13; 7:40286. PM ID: 28084321
Chen, C, et al. (2017) Mesenchymal stem cell transplantation in tight-skin mice identifies miR-151-5p as a therapeutic target for systemic sclerosis.
Cell Res.
. 2017 Apr 1; 27(4):559-577. PM ID: 28106077
Meyer, C, et al. (2017) Pseudotyping exosomes for enhanced protein delivery in mammalian cells..
Int J Nanomedicine
. 2017 May 1; 12:3153-3170. PM ID: 28458537
Domenis, R, et al. (2017) Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis.
Mediators Inflamm.
. 2017 Jun 21; 2017:4814987. PM ID: 28634420
Smith, JA & Daniel, R. (2016) Human vaginal fluid contains exosomes that have an inhibitory effect on an early step of the HIV-1 life cycle.
AIDS
. 2016 Nov 13; 30(17):2611-2616. PM ID: 27536982
Wen, Z, et al. (2016) NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs.
J. Clin. Invest.
. 2016 May 2; 126(5):1953-67. PM ID: 27088800
Junker, K, et al. (2016) Extracellular Vesicles and Their Role in Urologic Malignancies..
Eur. Urol.
. 2016 Aug 1; 70(2):323-31. PM ID: 26924769
Raimondo, F, et al. (2016) Urinary proteomics for the study of genetic kidney diseases..
Expert Rev Proteomics
. 2016 Mar 2; 13(3):309-24. PM ID: 26698090
Mukherjee, K, et al. (2016) Revers
IBL
e HuR-microRNA binding controls extracellular export of miR-122 and augments stress response.
EMBO Rep.
. 2016 Aug 1; 17(8):1184-203. PM ID: 27402548
Rim, KT & Kim, SJ. (2016) Quantitative Analysis of Exosomes From Murine Lung Cancer Cells by Flow Cytometry.
J Cancer Prev
. 2016 Sep 1; 21(3):194-200. PM ID: 27722146
Nudel, K. (2016) Neisseria gonorrhoeae modulates epithelial cell responses via the induction and release of the inhibitor of apoptosis protein cIAP2 in exosomes.
Thesis
. ;. Link: Thesis
Peterson, MF, et al. (2015) Integrated systems for exosome investigation.
Methods
. 2015 Oct 1; 87:31-45. PM ID: 25916618
Bhattarai, N, et al. (2015) Conserved Motifs within Hepatitis C Virus Envelope (E2) RNA and Protein Independently Inhibit T Cell Activation.
PLoS Pathog.
. 2015 Sep 1; 11(9):e1005183. PM ID: 26421924
Nudel, K, Massari, P & Genco, CA. (2015) Neisseria gonorrhoeae Modulates Cell Death in Human Endocervical Epithelial Cells through Export of Exosome-Associated cIAP2.
Infect. Immun.
. 2015 Sep 1; 83(9):3410-7. PM ID: 26077759
Bednarczyk, M, Biry?o, M & DAWIDOWICZ, A. (2015) Modern Geodetic Techniques in Spatial Measurement.
Thesis
. ;. Link: Thesis
Di Noto, G, et al. (2014) Immunoglobulin Free Light Chains and GAGs Mediate Multiple Myeloma Extracellular Vesicles Uptake and Secondary NfκB Nuclear Translocation.
Front Immunol
. 2014 Nov 11; 5:517. PM ID: 25386176
Jia, S, et al. (2014) Emerging technologies in extracellular vesicle-based molecular diagnostics.
Expert Rev. Mol. Diagn.
. 2014 Apr 1; 14(3):307-21. PM ID: 24575799
Santana, SM, et al. (2014) Cancerous epithelial cell lines shed extracellular vesicles with a bimodal size distribution that is sensitive to glutamine inhibition.
Phys Biol
. 2014 Nov 26; 11(6):065001. PM ID: 25426818
Basu, S & Bhattacharyya, SN. (2014) Insulin-like growth factor-1 prevents miR-122 production in neighbouring cells to curtail its intercellular transfer to ensure proliferation of human hepatoma cells.
Nucleic Acids Res.
. 2014 Jun 1; 42(11):7170-85. PM ID: 24813441