商品编号


30223-1



品牌


Lucigen



公司


Lucigen



公司分类


Polymerases




25,000 U


商品信息



Reliable transcription.





T7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity.






Specifications

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.

Source:
Purified from a strain of
E. coli
that expresses the recombinant T7 RNA Polymerase gene.



Unit Concentration

50,000 U/mL



Purity (SDS-PAGE)

>99%



SS Exonuclease

500 U<0.1% released



DS Exonuclease

500 U<0.1% released



Endonuclease

500 U<10% converted



RNAse Contamination

500 U = none detected



E. coli
16S rDNA Contamination

500 U<10 copies



Storage

-20°C



ORDER INFORMATION
Supplied at 50,000 U/mL in 50 mM Tris-HCl, 100 mM NaCl, 1 mM dithiothreitol, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9 @ 25°C. Also provided is 10X T7 RNA Polymerase Buffer whichin 1X form is composed of 40 mM Tris-HCl, 6 mM MgCl
2
, 10 mM Dithiothreitol, 2 mM Spermidine, pH 7.9 @ 25°C. 125,000 U is supplied as 5 × 25,000 U.