商品编号


LS000150



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

10 mg

商品信息
LactoperoxidaseChromatographically purified. A lyophilized powder.Store at -20°C.≥35 units per mg dry weight ABTS
Source:
Bovine Milk
I.U.B.: 1.11.1.7
Lactoperoxidase (LPO) is a glycoprotein with a hemin prosthetic group which may occur as a mixture of two isozymes. It has a molecular weight of 77,500 daltons. LPO catalyzes the hydrogen peroxide oxidation of iodide according to the following reaction:
2I
-
+ H
2
O
2
+ 2H
+
→ I
2
+ 2H
2
O.
Iodide reacts directly with the heme group; upon addition of H
2
O
2
the complex iodinates the substrate. LPO is inhibited by hydrazines. The assay procedure has been updated from that of Morrison to an ABTS
?
/H
2
O
2
based method with increased sensitivity and reproducibility.
Unit Definition
: One Unit reduces one micromole of hydrogen peroxide per minute at 25°C, pH 6.0. Note: This unit definition is based upon a new assay method. The previous method yielded approximately 2.8 times higher results.