商品编号


TG2004H-1



品牌


TopoGEN



公司


TopoGEN



公司分类


Human tdp1



Size

250 Units


商品信息


TopoGEN
provides purified Human Tyrosyl DNA Phosphodiesterase (Tdp1) purified to homogeneity to a single 68 kDa band on SDS-PAGE.? Tdp1 is catalytically active and will remove a terminal 3’ phosphotyrosyl group from a specifically designed oligo target site (oligo-Tyr).? Additionally,
TopoGEN
scientists have demonstrated that Tdp1 will release topo I covalently attached to genomic DNA isolated from cells treated with camptothecin using
TopoGEN
’s In Vivo Link Kit (TG1021).? Tdp1 is thought to be important in repair of topo/DNA adducts that form following topo-active drug therapy; therefore, there is considerable interest in identifying drugs that target Tdp1.? Such drugs may work well in combination with topo targeting strategies.

Figure 1.? SDS-PAGE of
TopoGEN
Purified Tdp1.? The gel was intentionally overloaded with 20ug of proten to reveal minor breakdown products.




Unit Defintion:
One unit of Tdp1 can release the terminal Tyrosine from 20 ng of substrate (oligo-Tyr) in 30 min at 37° C.


Tdp1 Reaction:
Release of topo I from the covalent complex is shown below.




Tdp1 Assays:
Biochemical assays for Tdp1 can be carried out using an oligo nucleotide with a 3’ terminal tyrosine residue.? Release of tyrosine from a 5’ 32P end labeled oligo is easily measured using a denaturing polyacrylamide gel or DNA sequencing gels.?
TopoGEN
Provides a kit for Tdp1 assay (TG1004) that has key reagents and controls/protocols for setting up a complete assay for Tdp1.