商品编号


GKX-5010



品牌


ProZyme



公司


ProZyme, Inc



公司分类


Glyko Enzymes



Size

10 U

商品信息

Description

Releases non-reducing terminal α(1-2,3,6)-linked mannose from oligosaccharides.?The enzyme exhibits some kinetic preference for α(1-2,3)-linked mannose over α(1-6)-linked mannose.?

Source:

Jack bean.

Specificity:

The enzyme has broad specificity, cleaving α(1-2,3,6)-linked mannose, although some kinetic preference has been observed (α1-2, 6 > 3). The enzyme will not cleave a single α(1-6) linked mannose residue from core β-mannose, but will, however, remove a single α1-3 linked mannose from the core β-mannose.?By using enzyme concentrations of around 50 U/ml and extended incubation times (up to 18 hours) at 37°C, complete removal of all non-reducing terminal α linked mannose residues may be achieved.

To expedite glycan sequencing studies, the sluggish activity of GKX-5010 Jack Bean α mannosidase toward α(1-6)-linked mannose residues can be overcome by using the enzyme in combination with the α mannosidase from
Xanthomonas mannihotis
?which rapidly cleaves α(1-6) linkages. The
X. mannihotis
α-mannosidase?may inhibit the action of other mannosidases if a branched (α1-6) mannose is present in the substrate, so is typically added after incubation of the substrate with Jack bean α-mannosidase (GKX-5010).





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Figure 1:

Cleavage specificity for GKX-5010?α(1-2,3,6)-Mannosidase.

Ships with:

WS0095 GKX-5010 Incubation Buffer (500 mM sodium acetate, 10 mM zinc chloride (pH 5.0).

Reaction Buffer:
5X concentrated buffer which when diluted gives 100 mM sodium acetate pH 5.0 containing 2 mM Zn2+.

Formulation:

20 mM Tris-HCl, 20 mM NaCl (pH 7.5)
MW:
~190 kD

pH optimum/range:
4.5

Unit Definition:

One unit is defined as the amount of enzyme that will hydrolyze 1 μmole of pNP-α-mannopyranoside per minute at pH 4.5 and 37°C.

Size:
10 U (70 μl)

Concentration:
?≥ 150 U/ml

Product Code:
GKX-5010