商品编号


GK80090



品牌


ProZyme



公司


ProZyme, Inc



公司分类


Glyko Enzymes



Size

50 mU

商品信息

Description

Releases unsubstituted, Ser/Thr-linked GalGalNAc from proteins; used with additional enzymes to remove more complex O-linked structures. Also known as?Endo-O-Glycosidase?, Endo-α N-acetylgalactosaminidase, O-Glycosidase.?

Source:

Recombinant gene from
Streptococcus pneumoniae
, expressed in
E.coli
.

Specificity:

The enzyme is highly specific, liberating Galβ1-3GalNAc from serine or threonine residues alone or as part of glycopeptides or glycoproteins. There is no apparent preference for serine over threonine. Substitution of the disaccharide core with sialic acid, fucose, N-acetylglucosamine or N-acetylgalactosamine residues, prevents cleavage of the glycosylamine linkage. Absence of the galactose residue or the acetamido group on the innermost N-acetylgalactosamine residue also prevents cleavage of the glycosylamine linkage.

O-Glycanase is included in our?Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans (
GK80110
), which may be used in conjunction with our?prO-LINK Extender? Kit for Complex O-Linked Glycans (
GK80115
).

Applications:


Detection of O-glycosylation in proteins

Epitope and binding site analysis

Bioactivity in O-glycans

Analysis of biosynthesis of glycoproteins

Structural analysis of O-glycan

Glycoprotein deglycosylation


Ships with:
WS0059 5X reaction buffer A [250 mM sodium phosphate pH 5.0]

Formulation:

20 mM Tris HCl pH 7.5 containing 25 mM NaCl.
Reaction Buffer:
5X concentrated buffer which when diluted gives 50 mM sodium phosphate pH 5.0.

MW:

~180 kD
pH optimum/rang
e:
5.5 / 5.0 - 7.0

Unit Definition:

One unit is defined as the amount of enzyme required to catalyze the release of 1 μmole of Galβ1-3GalNAcα1-p-nitrophenol per minute at 37°C, pH 5.5.

Size:
50 mU (40 ?l)

Concentration:
?≥ 1.25 U/ml

Product Code:
GK80090