商品编号


GKE-5008



品牌


ProZyme



公司


ProZyme, Inc



公司分类


Glyko Enzymes



Size

6 mU

商品信息

Description

Cleaves within the chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-glycans.?Endoglycosidase F2,?endo-beta-N-acetylglucosaminidase.?

Source:
Recombinant gene from
Elizabethkingia
meningosepticum
, expressed in
E. coli
. The source organism was previously known as?
Chryseobacterium [Flavobacterium] meningosepticum
?1
.?

Specificity:
The enzyme cleaves within the di-N-acetyl chitobiose ?(GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-linked glycans of oligosaccharide, glycopeptide or glycoprotein substrates. A single GlcNAc residue remains attached to the molecule after hydrolysis, the rest of the glycan is released. This specificity is distinct from PNGase F (GKE-5006), which cleaves between asparagine and the innermost GlcNAc of N-linked glycans. EndoF2 cleaves between GlcNAc-GlcNac in?biantennary glycans at a rate approximately 20 times higher than high mannose glycans. The enzyme is not active towards hybrid type or tri- and tetraantennary oligosaccharides.





?





Figure 1:

Cleavage specificity for GKE-5008?Endo F2.

R
1
: N & C substitution other than H
R
2
:?may be H or the rest of an oligosaccharide
R
3
:?may be H or?
α(1-6)-linked fucose

Applications:
Selective release of high mannose and biantennary complex type N-linked oligosaccharides from glycopeptides/ glycoproteins.

Ships with:
WS0161 5X Reaction Buffer, which when diluted gives 200 mM sodium acetate pH 4.5.

Formulation:
10 mM sodium acetate pH 4.5, 25 mM NaCl, 0.02% sodium azide

pH optimum:
4.5

Unit Definition:
One milliunit is defined as the amount of enzyme that will hydrolyze 1 nmole of the dansylated porcine fibrinogen glycopeptide substrate DnsVGEN(CHO)R per minute at pH 4.5 and 37°C.

Size:?
6 mU (60 ?l)

Concentration:
?≥ 100 mU/ml

Product Code:?
GKX-5008

1. Kim KK, Kim MK, Lim JH, Park HY, Lee ST.?Transfer of
Chryseobacterium meningosepticum
and
Chryseobacterium miricola
to
Elizabethkingia
gen. nov. as
Elizabethkingia meningoseptica
comb. nov. and
Elizabethkingia miricola
comb. nov.?
Int J Syst Evol Microbiol.
2005 May;55(Pt 3):1287-93.