商品编号


GKE-5002



品牌


ProZyme



公司


ProZyme, Inc



公司分类


Glyko Enzymes



Size

30 mU

商品信息

Description

Cleaves within the N,N'-diacetylchitobiose (GlcNAc-GlcNAc) core of high mannose and some hybrid type N-glycans.?Endoglycosidase H, mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase.?

Source:
Recombinant gene from
Streptomyces plicatus
1
, expressed in
E. coli
.?

Specificity:
Cleaves between the GlcNAc residues of the chitobiose (GlcNAc-GlcNAc)?core of N-linked glycans. Oligomannose & some hybrid type N-glycans are released from glycoproteins, leaving a single GlcNAc attached to asparagine. This specificity is distinct from Endo F2 (GKE-5008) which also cleaves biantennary complex type N-glycans, and PNGase F (GKE-5006) which releases most N-glycans by cleaving?between the innermost GlcNAc and asparagine.





?





Figure 1:

Cleavage specificity for GKE-5002 Endo H.

R
1
: Man
2-150
R
2
:?H, monosaccharide or oligosaccharide at C2 and/or C4
R
3
:?GlcNAc ± Fuc, Asn or Dol-pp

Applications:
Selective release of high mannose
N-
linked oligosaccharides from glycopeptides/ glycoproteins.?The enzyme has found extensive use in the characterization of glycoproteins, dolichol-linked glycans and the biosynthetic pathway for N-glycosylation.

Ships with:
?


WS0079 5x Reaction Buffer for GKE-5002 (500 mM Sodium Citrate/Phosphate, pH 5.5)?

WS0012 Denaturation Solution (2% SDS/1M βME)

WS0013 Detergent Solution (15% Detergent Solution)


Formulation:
50 mM sodium phosphate pH 7.5, 25 mM EDTA, ?and 0.05% sodium azide

pH optimum:
5.5

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 60 μmoles of denatured ribonuclease B in 1 hour at 37°C, pH 5.5.

Size:
30 mU (200 ?l)

Concentration:
?≥ 0.15 U/ml

Product Code:
?GKE-5002

1. Robbins PW, Trimble RB, Wirth DF, Hering C, Maley F, Maley GF, Das R, Gibson BW, Royal N, Biemann K.?Primary structure of the
Streptomyces
enzyme endo-beta-N-acetylglucosaminidase H.?
J Biol Chem.
1984 Jun 25;259(12):7577-83.