商品编号


GKE-5020D



品牌


ProZyme



公司


ProZyme, Inc



公司分类


Glyko Enzymes



Size

1 U

商品信息

Description

PNGase F, peptide-
N
-glycosidase F,
peptide-
N
4
-(
N-
acetyl-β-glucosaminyl)asparagine amidase.
?Releases intact N-glycans by cleaving
?between the innermost GlcNAc and Asn.?The same formulation provided in GlykoPrep?,?≥ 10 U/ml and EDTA-free.

Source:
Recombinant gene from
Elizabethkingia
meningosepticum
, expressed in
E. coli
. The source organism was previously known as?
Chryseobacterium [Flavobacterium] meningosepticum
[1].

Specificity:?
Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide-bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
?





?





Figure 1:

Cleavage specificity for GKE-5020 N-Glycanase (PNGase F).
R
1
: N & C substitution other than H
R
2
:?may be H or the rest of an oligosaccharide
R
3
:?may be H or?
α(1-6)-linked fucose

Applications:?
To obtain efficient deglycosylation of glycoprotein substrates under non-denaturing conditions, it is necessary?to use a higher starting concentration of enzyme. N-Glycanase? PLUS and ULTRA (EDTA-Free) are supplied at ≥ 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller?reaction volumes and shorter reaction times to be explored.

"B" pack size ships with:



1 ea WS0010 5x N-Glycanase Reaction Buffer

1 ea WS0012 Denaturation Solution

1 ea WS0013 Detergent Solution.



Buffers do not ship with "D" pack size.?




Formulation:
20 mM Tris HCl pH 7.5, 50 mM NaCl

pH optimum:
8.6

pH range:
7.5 - 9.5

Unit Definition:

One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5.

Size:
1 U in 100 ?l

Concentration:
?≥ 10 U/ml

Product Code:
GKE-5020D

Not formulated for FACE? Analysis, use GK50052.

1. Kim KK, Kim MK, Lim JH, Park HY, Lee ST.?Transfer of
Chryseobacterium meningosepticum
and
Chryseobacterium miricola
to
Elizabethkingia
gen. nov. as
Elizabethkingia meningoseptica
comb. nov. and
Elizabethkingia miricola
comb. nov.?
Int J Syst Evol Microbiol.
2005 May;55(Pt 3):1287-93.