商品编号


GKE-5010B



品牌


ProZyme



公司


ProZyme, Inc



公司分类


Glyko Enzymes



Size

400 mU

商品信息

Description

PNGase F, peptide-
N
-glycosidase F,
peptide-
N
4
-(
N-
acetyl-β-glucosaminyl)asparagine amidase.
?Releases intact N-glycans by cleaving
?between the innermost GlcNAc and Asn.

Source:
Recombinant gene from
Elizabethkingia
meningosepticum
, expressed in
E. coli
. The source organism was previously known as?
Chryseobacterium?Chryseobacterium [Flavobacterium] meningosepticum
[1].

Specificity:?
Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide-bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
?





?





Figure 1:

Cleavage specificity for GKE-5010 N-Glycanase (PNGase F).
R
1
: N & C substitution other than H
R
2
:?may be H or the rest of an oligosaccharide
R
3
:?may be H or?
α(1-6)-linked fucose

Applications:?
To obtain efficient deglycosylation of glycoprotein substrates under non-denaturing conditions, it is necessary?to use a higher starting concentration of enzyme. N-Glycanase? PLUS and ULTRA (EDTA-Free) are supplied at ≥ 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller?reaction volumes and shorter reaction times to be explored.

Ships with:
?



1ea WS0010 5x N-Glycanase Reaction Buffer:100 mM sodium phosphate pH 7.5, 0.1% sodium azide

1ea WS0012 Denaturation Solution:?2% SDS, 1 M 2-mercaptoethanol

1ea WS0013 Detergent Solution: 15% Detergent

1ea WS0145 5x N-Glycanase Tris Reaction Buffer: 50mM Tris-HCl pH 8.0



Also available in
"D" (1 U)
pack size, which does not ship with buffers.

Formulation:
20 mM Tris HCl pH 7.5, containing 1 mM EDTA and 50 mM NaCl

pH optimum:
8.6

pH range:
7.5 - 9.5

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1?μmole of denatured ribonuclease B per minute?at pH 7.5 and 37°C.

Size:
400 mU (40 ?l)

Concentration:
?≥ 10 U/ml

Product Code:
GKE-5010B

Not formulated for FACE? Analysis, use GK50052.

1. Kim KK, Kim MK, Lim JH, Park HY, Lee ST.?Transfer of
Chryseobacterium meningosepticum
and
Chryseobacterium miricola
to
Elizabethkingia
gen. nov. as
Elizabethkingia meningoseptica
comb. nov. and
Elizabethkingia miricola
comb. nov.?
Int J Syst Evol Microbiol.
2005 May;55(Pt 3):1287-93.