商品编号


GKE-5006D



品牌


ProZyme



公司


ProZyme, Inc



公司分类


Glyko Enzymes



Size

1 U

商品信息

Description

PNGase F, peptide-
N
-glycosidase F,
peptide-
N
4
-(
N-
acetyl-β-glucosaminyl)asparagine amidase.
?Releases intact N-glycans by cleaving
?between the innermost GlcNAc and Asn.?

Source:
Recombinant gene from
Elizabethkingia
meningosepticum
, expressed in
E. coli
. The source organism was previously known as?
Chryseobacterium [Flavobacterium] meningosepticum
[1].

Specificity:?
Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide-bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free.?





?





Figure 1:

Cleavage specificity for GKE-5006 N-Glycanase (PNGase F).
R
1
: N & C substitution other than H
R
2
:?may be H or the rest of an oligosaccharide
R
3
:?may be H or?
α(1-6)-linked fucose

Applications:


Release of intact N-linked glycans from glycopeptides and glycoproteins

Structure-function studies of N-glycosylated glycoproteins

Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies


Supplied at a concentration of?≥ 2.5 U/ml. For a more concentrated formulation of?≥ 10?U/ml, which may be useful for native digestions, see
GKE-5010B
or
GKE-5020B
(EDTA-free).

"D" pack size does not ship with reaction buffers?that ship with
"A"
?(100 mU)?and
"B"
?(200 mU) pack sizes. Please
contact us
if you require buffers.


Formulation:

20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5)
Reaction Buffer:

5X concentrated buffer, when diluted gives 20 mM sodium phosphate pH 7.5, containing 0.02% sodium azide.
MW:

~35,000 daltons
pH optimum/range
:
8.6 / 7.5 - 9.5
Unit Definition:

One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5.

Not formulated for FACE? analysis, use GK50050 instead.

Size:
1 U in 400 ?l

Concentration:
?≥ 2.5 U/ml

Product Code:
GKE-5006D

1. Kim KK, Kim MK, Lim JH, Park HY, Lee ST.?Transfer of
Chryseobacterium meningosepticum
and
Chryseobacterium miricola
to
Elizabethkingia
gen. nov. as
Elizabethkingia meningoseptica
comb. nov. and
Elizabethkingia miricola
comb. nov.?
Int J Syst Evol Microbiol.
2005 May;55(Pt 3):1287-93.