商品编号


TG2028-1



品牌


TopoGEN



公司


TopoGEN



公司分类


Dna molecular weight markers



Size

10 ug

商品信息


Linear pRYG DNA
Marker
Description
pRYG is a pUC19 derivative in which the 206 bp SphI-NarI fragment of pUC19 was replaced with a 245 bp SphI-AccI fragment upstream of the human adult beta-globin gene promoter. The 245 bp fragment contains a 54 bp alternating repeat of purine/pyrimidine sequence that constitutes a strong eukaryotic topo II cleavage site.? Cleavages within the repeat are re
ADI
ly detected in the absence of topo II inhibitors such as m-AMSA or VM-26.? The sequence of the plasmid is given in Table 2 of Spitzner et al.



Reference:
For detailed description of pRYG, its reactivity with Topo II and the sequence, see Spitzner et al., Nuc. Acid. Res. 18: 1-11 (1990)