商品编号


JMAd-43



品牌


Seven Hills Bioreagent



公司


Seven Hills Bioreagent,inc



公司分类


adenoviral-vectors



Size

0.5 ml

商品信息

Category
MAP Kinase Related

Gene
p38beta DN

Vector Construction
Dominant negative AGF mutation in human p38beta MAPK with 5’ flag epitope was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the human p38beta MAPK expression cassette, generating a replication deficient recombinant virus.

Supplied As
Crude HEK293 cell lysate.

Storage
Store at -80°C. Multiple freeze/thaw cycles not recommended.

Epitope Tag
Flag

QC Testing
Western Blot (to block endogenous p38 phos)

Titer
Approx 1E8/ml