商品编号


JMAd-21



品牌


Seven Hills Bioreagent



公司


Seven Hills Bioreagent,inc



公司分类


adenoviral-vectors



Size

0.5 ml

商品信息

Category
Transcription Factors

Gene
GATA4-engrailed dominant negative fusion (GATA4 211-329 – engr 2-298)

Vector Construction
Mouse Gata4
CDN
A for amino acids 211-329 fused to amino acids 2-298 of engrailed to make a dominant negative Gata protein. It contains 5’ flag epitope. This cassette was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the
CDN
A expression cassette, generating a replication deficient recombinant virus.

Supplied As
Crude HEK293 cell lysate.

Storage
Store at -80°C. Multiple freeze/thaw cycles not recommended.

Epitope Tag
Flag

QC Testing
Western Blot

Titer
Approx 1E8/ml