商品编号


JMad-08



品牌


Seven Hills Bioreagent



公司


Seven Hills Bioreagent,inc



公司分类


adenoviral-vectors



Size

0.5 ml

商品信息

Category
Calcineurin/NFAT

Gene
Constitutively nuclear NFATc3 (amino acids 282-1068 of nuclear factor of activated T-cells) Human
CDN
A fragment with 5’ flag tag

Vector Construction
Human
CDN
A for amino acids 282-1068 of NFATc3 (nuclear factor of activated T-cells) [NFAT4] with 5’ flag tag was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the human
CDN
A fragment with 5’ flag tag expression cassette, generating a replication deficient recombinant virus.

Supplied As
Crude HEK293 cell lysate.

Storage
Store at -80°C. Multiple freeze/thaw cycles not recommended.

Epitope Tag
None

QC Testing
Western Blot

Titer
Approx 1E8/ml