商品编号


JRAd-110



品牌


Seven Hills Bioreagent



公司


Seven Hills Bioreagent,inc



公司分类


adenoviral-vectors



Size

0.5 ml

商品信息

Category
Contractile Proteins

Gene
cMyBP-C
Phos-mutant

Vector Construction
Wild-type cMyBP-C
CDN
A was subjected to site directed mutagenesis in which the PKA/CaMKII/PKC sites (Ser-273, -282 and -302) were mutated to Alanine (cMyBP-C
Phos-mutant
) to mimic constitutive dephosphorylation (phospho-ablation). cMyBP-C
Phos-mutant

CDN
A was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17, which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the mouse
CDN
A expression cassette, generating a replication deficient recombinant virus.

Supplied As
Crude HEK293 cell lysate.

Storage
Store at -80°C. Multiple freeze/thaw cycles not recommended.

Epitope Tag
A myc-tag EQKLISEEDL is placed after the initiator Met (first ATG) codon

QC Testing
Western Blot - see datasheet

Titer
Approx 1E8/ml