商品编号


F1200



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Inhibitors



Size

5 mg


商品信息

Bortezomib; VELCADE; PS3PS-341 is a specific, revers
IBL
e and cell permeable dipeptide boronic acid inhibitor of the chymotrypsin-like activity of the proteasome (Ki=0.6nM). It inhibits proliferation of various tumor cell lines with nanomolar potency. Its commercial version was approved by the USFDA for the treatment of relapsed multiple myeloma. The typical concentrations for cell culture use are 1-20 ?M. For in vitro use, the typical concentrations are 10-100 ?M. 41; MG-341; MG341; Pyz-Phe-boroLeu; CAS# 179324-69-7

Additional Information






Product Name:

PS-341



Also Known As:

Bortezomib; VELCADE; PS341; MG-341; MG341; Pyz-Phe-boroLeu; CAS# 179324-69-7



Catalog No.:

F1200



Size:

5 mg



Molecular Weight:

384.2 Da



Species:

N/A



Source:

Synthetic



Stock:

Powder



Concentration:

N/A



Quality Assurance:

>98% by HPLC and NMR



Storage:

Elig
IBL
e for room temperature shipping. Store at -80°C upon receiving; avoid multiple freeze-thaw cycles after dissolving in DMSO.



PDF Data Sheet:

Download PDF datasheet



NCBI RefSeq:

N/A



Image(s):

No



Shipping Method:

Room temperature shipping



References:

1. Yi-He Ling
et al
. (2002) Mol Cancer Ther, 1:841-849.
2. Adams J. (2002) The Oncologist, 7:9-16.
3. Ling, Y.,
et al
. (2003).Clin.Cancer Res.9,1145-1154
4. Chauhan, D.,
et al
. (2005) Cancer Cell 8: 407-419
5. Dudek SA
et al
. (2010) J Virol. 84:9439-51




Details

PS-341 is a specific, revers
IBL
e and cell permeable dipeptide boronic acid inhibitor of the chymotrypsin-like activity of the proteasome (Ki=0.6nM). It inhibits proliferation of various tumor cell lines with nanomolar potency. Its commercial version was approved by the USFDA for the treatment of relapsed multiple myeloma. The typical concentrations for cell culture use are 1-20 ?M. For in vitro use, the typical concentrations are 10-100 ?M.

Images
(Click image to enlarge)

100 nM bovine 26S proteasome (Cat. # A1200) was incubated with DMSO or with 10 ?M PS-341 for 10 min at 37 °C in 20 mM Tris, pH 7.1, 50 mM NaCl, 2 mM ATP, 5 mM MgCl2, 2 mM bME and 10% glycerol. The proteasome was then diluted 10X into 50 ?M SUC-LLVY-AMC (Cat. # G1100) in a buffer containing 20 mM Tris, pH 7.1, 2 mM bME. The released AMC fluorescence was monitored by a plate reader.

Equal amount of whole cell lysates prepared from DMSO (lane 1) or 20 μM PS-341 (lane 2)-treated HEK293T cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. Cells were treated with DMSO or PS-341 for 4 hours.