商品编号


F1100



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Inhibitors



Size

5 mg




商品信息

MG-132 is a revers
IBL
e proteasome inhibitor that belongs to the family known as peptide aldehydes. MG-132 is cell permeable and can be used to treat various mammalian cells to block proteasome activities. MG-132 can be dissolved in DMSO for stock solution up to 100 mM. The typical concentrations for cell culture use are 1-10 ?M. For in vitro use, the typical concentrations are 50-100 ?M.MG132, Z-LLL-al, Z-Leu-Leu-Leu-CHO, CAS#: 133407-82-6

Additional Information






Product Name:

MG-132



Also Known As:

MG132, Z-LLL-al, Z-Leu-Leu-Leu-CHO, CAS#: 133407-82-6



Catalog No.:

F1100



Size:

5 mg



Molecular Weight:

475.6 Da



Species:

N/A



Source:

Synthetic



Stock:

Powder



Concentration:

N/A



Quality Assurance:

>98% by HPLC and NMR



Storage:

Elig
IBL
e for room temperature shipping. Store at -80°C upon receiving; avoid multiple freeze-thaw cycles after dissolving in DMSO.



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

N/A



Image(s):

(Click image to enlarge)

100 nM bovine 26S proteasome (Cat. # A1200) was incubated with DMSO or with 10 ?M MG-132 for 10 min at 37 °C in 20 mM Tris, pH 7.1, 50 mM NaCl, 2 mM ATP, 5 mM MgCl2, 2 mM bME and 10% glycerol. The proteasome was then diluted 10X into 50 ?M SUC-LLVY-AMC (Cat. # G1100) in a buffer containing 20 mM Tris, pH 7.1, 2 mM bME. The released AMC fluorescence was monitored by a plate reader.

Equal amount of whole cell lysates prepared from DMSO (lane 1) or 20 μM MG132 (lane 2)-treated HEK293T cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. Cells were treated with DMSO or MG-132 for 4 hours.



Shipping Method:

Room temperature shipping



References:

1. Wilk S,
et al
. (1993) Enzyme Protein 47(4 – 6), 306 – 313.
2. Bush DT,
et al
. (1997) J Biol Chem 272(14), 9086 – 9092. 3. Myung J,
et al
. (2001 Med Res Rev 21(4), 245 – 273.




Details

MG-132 is a revers
IBL
e proteasome inhibitor that belongs to the family known as peptide aldehydes. MG-132 is cell permeable and can be used to treat various mammalian cells to block proteasome activities. MG-132 can be dissolved in DMSO for stock solution up to 100 mM. The typical concentrations for cell culture use are 1-10 ?M. For in vitro use, the typical concentrations are 50-100 ?M.

Images
(Click image to enlarge)

100 nM bovine 26S proteasome (Cat. # A1200) was incubated with DMSO or with 10 ?M MG-132 for 10 min at 37 °C in 20 mM Tris, pH 7.1, 50 mM NaCl, 2 mM ATP, 5 mM MgCl2, 2 mM bME and 10% glycerol. The proteasome was then diluted 10X into 50 ?M SUC-LLVY-AMC (Cat. # G1100) in a buffer containing 20 mM Tris, pH 7.1, 2 mM bME. The released AMC fluorescence was monitored by a plate reader.

Equal amount of whole cell lysates prepared from DMSO (lane 1) or 20 μM MG132 (lane 2)-treated HEK293T cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. Cells were treated with DMSO or MG-132 for 4 hours.