商品编号


4418-096-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


DNA Damage



Size

96 tests

商品信息

Description

4418-096-k_calculation_worksheet

In an
ELISA
assay, immobilized gamma-H2AX antibody in the wells of a 96-well plate captures gamma-H2AX from sample lysate.? Incubation with a H2AX detecting antibody, followed by addition of a goat anti-mouse HRP conjugate and a chemiluminescent HRP substrate yields relative light units (RLU) that directly correlates with the amount of gamma-H2AX in the sample. The gamma-H2AX polyclonal antibody may be purchased separately.

FEATURES:


Chemiluminescent, non-r
ADI
oactive
ELISA

Pre-coated 96 well capture antibody plate

Dynamic range from 10 pM to 800pM

Sensitivity with 5 pM of gamma-H2AX


APPLICATIONS:
Quantitation of gamma-H2AX levels in peripheral blood mononuclear cells, tissue culture cells, and tissue biopsies.




Catalog # 4418-096-K includes :



Catalog Number

Description

Qty



4418-096-01

Gamma-H2AX Standard, 1 ?M

1



4418-096-06

Jurkat Cell Lysate Control

1



4418-096-02

Assay Buffer

1



4418-096-05

Cell Lysis Reagent

1



4418-096-07

25X Wash Buffer

1



4418-096-P

96-Stripwell pre-coated plate with 3 film sealers

1



4675-096-01

PARP PeroxyGlow? A

1



4675-096-02

PARP PeroxyGlow? B

1



4418-096-03

H2AX IgM Detecting Antibody

1



4418-096-04

Goat anti-Mouse IgM HRP Conjugate

1




Abstract 2423: Development of a validated high throughput
ELISA
assay for gamma H2AX as a pharmacodynamic
Marker
Jay George and Hai Xu
Cancer Res., Oct 2014; 74: 2423.
http://cancerres.aacrjournals.org/cgi/content/abstract/74/19_Supplement/2423}

Engineered cellular gene-replacement platform for selective and induc
IBL
e proteolytic profiling
Charles W. Morgan, Juan E. Diaz, Samantha G. Zeitlin, Daniel C. Gray, and James A. Wells
PNAS, Jul 2015; 112: 8344 – 8349.
http://www.pnas.org/cgi/content/abstract/112/27/8344