商品编号


J1100



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E2 Conjugating Enzymes



Size

1 Kit

商品信息

The E2 Screening Kit is developed for identification of the E2 Ub-conjugating enzyme(s) for a specific E3 Ub ligase and/or a protein substrate.

Additional Information






Product Name:

E2 Screening Kit



Also Known As:

N/A



Catalog No.:

J1100



Size:

1 Kit



Molecular Weight:

N/A



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

90% - 95% based on SDS-PAGE



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF datasheet
,
MSD
S



NCBI RefSeq:

N/A



Image(s):

No



Shipping Method:

Dry ice shipping



References:

N/A




Details


Description
The E2 Screening Kit is developed for identification of the E2 Ub-conjugating enzyme(s) for a specific E3 Ub ligase and/or a protein substrate.

Components
? 20X UBE1 (2 ?M)......................................................100?l ? 10X E2 Ub conjugating enzymes
(29 E2s, see table below, 20?M)................................20 ?l each ? 10X Human Ubiquitin (500 ?M)................................200 ?l
? 10X Ubiquitination Buffer.............................................1 ml ? 40 mM ATP...............................................................250 ?l

E2 Name
1: 6XHis-Ube2A (Rad6A) 2: 6XHis-Ube2B
3: 6XHis-Ube2C(UbcH10 ) 4: 6XHis-Ube2D1 (UbcH5A)
5: 6XHis-Ube2D2 (UbcH5B) 6: 6XHis-Ube2D3 (UbcH5C)
7: 6XHis-Ube2D4 (UbcH5D) 8: 6XHis-Ube2E1 (UbcH6)
9: 6XHis-Ube2E2 (UbcH8) 10: 6XHis-Ube2E3 (UbcH9)
11: 6XHis-Ube2F 12: 6XHis-Ube2G1
13: 6XHis-Ube2G2 14: 6XHis-Ube2H (UbcH2)
15: 6XHis-Ube2I 16: 6XHis-Ube2J1
(1-282)
17: GST-Ube2K (E2-25K) 18: 6XHis-Ube2L3
19: 6XHis-Ube2L6 20: 6XHis-Ube2M (UbcH12)
21: 6XHis-UbeN (Ubc13/Uev1a) 22: 6XHis-Ube2Q2
23: 6XHis-Ube2R1 (Cdc34) 24: 6XHis-Ube2R2
25: 6XHis-Ube2S 26: 6XHis-Ube2T
27: 6xHis-Ubc13/UbE2V2 28: 6XHis-Ube2W
29: 6XHis-Ube2Z