商品编号


7501-500-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


Oxidative Damage



Size

480 Wells

商品信息

Description

7501-500-k_calculation_worksheet

In the HT Superoxide Dismutase Assay, superoxide r
ADI
cal ions, generated from the conversion of xanthine to uric acid, and hydrogen peroxide by xanthine oxidase (XOD), convert WST-1 to WST-1-formazan. WST-1-formazan absorbs light at 450 nm. SODs reduce superoxide ion concentrations and thereby lower the rate of WST-1 formazan formation. The extent of reduction in the appearance of WST-1 formazan is a measure of SOD activity present in experimental samples. This assay is free of interference by other catalytic activities, and unlike some other assay kits for SOD, this system is not greatly disturbed by trace metals.

FEATURES:


96 well format for high throughput screening

Assay performed in as little as 6 minutes

Suitable for mammalian tissues and cell lysates

Suitable for the assay of (Mn
2+
)-SOD, (Fe
2+
)-SOD, and (Cu/Zn)-SOD


APPLICATIONS:

Calculating relative SOD activity by measuring the percent inhibition of the rate of formation of WST-1 formazan.

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Catalog # 7501-500-K includes :



Catalog Number

Description

Qty



7501-500-01

SOD Standard

1



7501-500-03

Xanthine Oxidase (XOD)

1



7501-500-02

10X SOD Buffer

1



7501-500-04

10X Xanthine Solution

1



7501-500-05

20% Triton X-100

1



7501-500-06

WST-1 Reagent

1



7512-100-06

96-Well Plate

1




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