商品编号


4150-010-EB



品牌


Trevigen



公司


Trevigen, Inc



公司分类


DNA Damage



Size

10μg, 1ml, 50μg, 1ml

商品信息

Description

CONCENTRATION
10 or 50 ?g per vial

SOURCE
Purified from
E. coli
containing a recombinant plasmid harboring the
S. solfataricus
Dpo4 gene.

SUBSTRATE SPECIFICITY
Translesion synthesis across AP sites, cis-syn thymine-thymine dimer, 6-4 photoproducts, cisplatinum- and acetyl aminofluorene guanine adducts.

STORAGE
Store at -20°C in a manual defrost freezer. For long-term storage, freeze at -80°C in working aliquots.

APPLICATIONS
Allows for coupling to PCR for amplification of damaged DNA.




Catalog # 4150-050-EB includes :?



Catalog Number

Description

Qty



3900-500-15

10X REC? Buffer 15

1



4150-010-02

100 mM MgCl
2

1



4150-010-03

50 mM MnCl
2

1



4150-050-01

S. solfataricus Dpo4

1







Catalog # 4150-010-EB includes :



Catalog Number

Description

Qty



3900-500-15

10X REC? Buffer 15

1



4150-010-02

100 mM MgCl
2

1



4150-010-03

50 mM MnCl
2

1



4150-010-01

S. solfataricus
Dpo4

1




Structural and biochemical impact of C8-aryl-guanine adducts within the NarI recognition DNA sequence: influence of aryl ring size on targeted and semi-targeted mutagenicity
Michael Sproviero, Anne M.R. Verwey, Katherine M. Rankin, Aaron A. Witham, Dmitriy V. Soldatov, Richard A. Manderville, Mostafa I. Fekry, Shana J. Sturla, Purshotam Sharma, and Stacey D. Wetmore Nucleic Acids Res., Dec 2014; 42: 13405 – 13421.
Nucleic Acids Res., Dec 2014; 42: 13405 – 13421
http://nar.oxfordjournals.org/cgi/content/abstract/42/21/13405