商品编号


4677-096-K



品牌


Trevigen



公司


Trevigen, Inc



公司分类


DNA Damage



Size

96 tests

商品信息

Description

This
ELISA
based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a colorimetric HRP substrate yields relative absorbance that correlates with PARP-1 activity.

FEATURES:


Colorimetric readout

96 strip-well format

Sensitive – detects 10 mU PARP /well

Assay Time ~3 hrs


APPLICATIONS:
For the
in vitro
screening of candidate PARP-1 inhibitors and determination of IC50 values.




Catalog # 4677-096-K includes :



Catalog Number

Description

Qty



4667-50-03

3-Aminobenzamide

1



4668-050-01

PARP-HSA, 10 Units/?l

1



4671-096-02

20X PARP Buffer

2



4671-096-03

10X PARP Cocktail

1



4671-096-06

10X Activated DNA

1



4671-096-04

10X Strep-Diluent

1



4677-096-P

Histone-Coated Plate Natural Strip Well Plate

1



4800-30-06

Strep-HRP

1



4822-96-08

TACS-Sapphire?

1




Effect of Small-Molecule Modification on Single-Cell Pharmacokinetics of PARP Inhibitors
Greg M. Thurber, Thomas Reiner, Katherine S. Yang, Rainer H. Kohler, and Ralph Weissleder
Mol. Cancer Ther., Apr 2014; 13: 986 – 995
http://mct.aacrjournals.org/cgi/content/abstract/13/4/986

Combinatorial Effects of PARP Inhibitor PJ34 and Histone Deacetylase Inhibitor Vorinostat on Leukemia Cell Lines
EWA JASEK, MARIUSZ GAJDA, GRZEGORZ J. LIS, MA?GORZATA JASI?SKA, and JAN A. LITWIN
Anticancer Res, Apr 2014; 34: 1849 – 1856.
http://ar.iiarjournals.org/cgi/content/abstract/34/4/1849

Silencing of poly(ADP-ribose) polymerase-1 suppresses hyperstretch-induced expression of inflammatory cytokines in vitro
Wang, J., Liu, L., Xia, Y. and Wu, D.
Acta Biochim Biophys Sin, May 2014; 10.1093/abbs/gmu035.
http://abbs.oxfordjournals.org/cgi/content/abstract/gmu035v1

New Insights into PARP Inhibitors’ Effect on Cell Cycle and Homology- Directed DNA Damage Repair
Petar Jelinic and Douglas A. Levine
Mol. Cancer Ther., Jun 2014; 13: 1645 – 1654.
http://mct.aacrjournals.org/cgi/content/abstract/13/6/1645

Disruption of Wnt/β-Catenin Signaling and Telomeric Shortening Are Inextricable Consequences of Tankyrase Inhibition in Human Cells
“Ozlem Kulak, Hua Chen, Brody Holohan, Xiaofeng Wu, Huawei He, Dominika Borek, Zbyszek
Otwinowski, Kiyoshi Yamaguchi, Lauren A. Garofalo, Zhiqiang Ma, Woodring Wright, Chuo Chen, Jerry W.Shay, Xuewu Zhang, and Lawrence Lum”
Mol. Cell. Biol., Jul 2015; 35: 2425 – 2435.
http://mcb.asm.org/cgi/content/abstract/35/14/2425

Acyl-CoA-binding domain containing 3 modulates NAD+ metabolism through activating poly(ADP-ribose) polymerase 1
Yong Chen, Sookhee Bang, Soohyun Park, Hanyuan Shi, and Sangwon F. Kim
Biochem. J., Jul 2015; 469: 189 – 198.
http://www.biochemj.org/cgi/content/abstract/469/2/189