商品编号


C3100



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E2 Conjugating Enzymes



Size

25 ?g


商品信息

Ube2M is an E2 enzyme, which is part of the E1, E2, and E3 cascade respons
IBL
e for conjugation of the Ub-like protein NEDD8 to protein substrates. This E2 is unique from Ube2F in the fact that it does not need to complex with an E3 in order to conjugate the NEDD8 to a Cullin Ring Ligase (CRL), which is a subclass of Ub E3s. The major CRL for Ube2M is cullin-4A.

Additional Information






Product Name:

6xHis-Ube2M (UbcH12)



Also Known As:

UBC12; hUbc12; UBC-RS2



Catalog No.:

C3100



Size:

25 ?g



Molecular Weight:

20.9 kDa



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~95% by SDS-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

NM_003969



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-Ube2M (UbcH12)







Shipping Method:

Dry ice shipping



References:

1. Osaka F,
et al
. (1998) Genes Dev 12(15), 2263 – 2268.
2. Gong L,
et al
. (1999) J Biol Chem 274(17), 12036 – 12042. 3. Huang DT,
et al
. (2009) Mol Cell 33, 483–495.
4. Huang AM,
et al
. (2011) Biochemical Pharmocology 82, 35 – 42.




Details

Ube2M is an E2 enzyme, which is part of the E1, E2, and E3 cascade respons
IBL
e for conjugation of the Ub-like protein NEDD8 to protein substrates. This E2 is unique from Ube2F in the fact that it does not need to complex with an E3 in order to conjugate the NEDD8 to a Cullin Ring Ligase (CRL), which is a subclass of Ub E3s. The major CRL for Ube2M is cullin-4A.

Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-Ube2M (UbcH12)