商品编号


R300381,



品牌


Rubicon Genomics



公司


Rubicon Genomics



公司分类


Products



Size

1 Ea







商品信息

.detail-txt img { max-width: 100%; }







Highly Reproduc
IBL
e CNV Detection Over the Entire Genome

Amplified libraries from 11 individual flow-sorted H929 cells were sequenced on an
Illumina
MiSeq? and downsampled to 250,000 total reads. Thirty-five base single-end reads were mapped to human over the entire genome.








Highly Reproduc
IBL
e CNV Detection in Chromosome 1

In the each of the individual H929 cells (see previous slider), a 22 Mb loss and 30 Mb gain were consistently detected in chromosome 1 as indicated by the pink or blue bars.








PicoPLEX DNA-seq Kit Provides Aneuploidy Calls Concordant with Arrays

PicoPLEX DNA-seq Kit was tested for equivalency to 24sure? arrays (
Illumina
) by using the same single embryo cell library for array and NGS testing. Libraries were pooled in groups with 48 compat
IBL
e bar codes, and sequenced in a 2x100 MiSeq v2 PE experiment. Sequencing results were concordant with array results and sequencing showed a terminal gain in 2p (shown in figure) and a terminal loss in 12q (data not shown), which were consistent with an undetected translocation in the paternal DNA confirmed by high resolution FISH analysis. (Data provided by GIVF).








Individual Library Quantification Eliminated

Flow-sorted unsynchronized H929 cells were amplified, pooled with constant volume, and loaded onto a MiSeq v3 flow cell. Quantification of the libraries was unnecessary before pooling due to the highly reproduc
IBL
e amount of product produced by PicoPLEX DNA-seq reactions. The columns which provided no reads were wells in which the cell was absent.








PicoPLEX DNA-seq Kit Library Preparation Workflow for
Illumina
NGS Platforms









PicoPLEX DNA-seq Kit Single-Tube Workflow









PicoPLEX DNA-seq Kit Technology

PicoPLEX DNA-seq Kit uses the same technology as the WGA kit: cells are lysed, quasi-random primers pre-amplify the DNA selectively, and a final PCR amplification adds the
Illumina
barcodes.




Store at -20 °C.

Guaranteed for 12 months at -20°C in a constant temperature freezer.


CAT. NO. R300381, 48 reactions

The kit contains everything needed to do 48 reactions, including 48 dual indexes in a single use 96-well plate.


Reduce ambiguity
: highly reproduc
IBL
e CNV and aneuploidy detection



Reduce workflow
: from a single cell to a sequencing-ready library in three steps



Reduce cost
: a single kit contains everything needed to prepare a sequencing-ready library



Reduce contamination and error
: library prep in a single tube or well, no transfers necessary



Reduce time to results
:
Illumina
NGS libraries prepared in less than 3 hours