商品编号


C1901



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E2 Conjugating Enzymes



Size

100 ?g


商品信息

Ube2E2 is an E2 enzyme, which is part of the E1, E2, and E3 cascade respons
IBL
e for ubiquitination of protein substrates. It shares 96% identical amino acid sequence with Ube2E1 and Ube2E3 with the exception of the N-terminal extension that has little in common with the other two E2s. Ube2E2 interacts with ARA54 and RNF8. The RNF8/ Ube2E2 interaction is found to catalyze K48-linked poly-ubiquitination. Ube2E2 may play a role in ubiquitination and subsequent proteasomal degradation of the E3 Ub ligase Parkin.

Additional Information






Product Name:

6xHis-Ube2E2 (UbcH8)



Also Known As:

UBCH8



Catalog No.:

C1901



Size:

100 ?g



Molecular Weight:

22.3 kDa



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~90% by SDS-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

NM_152653



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified6xHis-Ube2E2 (UbcH8)







Shipping Method:

Dry ice shipping



References:

1. Kimura M,
et al
. (1997) Cytogenet Cell Genet 78(2), 107 – 111.
2. Zhang Y,
et al
. (2000) Proc Natl Acad Sci USA 97(24), 13354 – 13359. 3. Ito K,
et al
. (2001) European Journal of Biochemistry 268, 2725–2732.
4. Lok GTM,
et al
. (2012) Nucleic Acids Res 40(1), 196-205.




Details

Ube2E2 is an E2 enzyme, which is part of the E1, E2, and E3 cascade respons
IBL
e for ubiquitination of protein substrates. It shares 96% identical amino acid sequence with Ube2E1 and Ube2E3 with the exception of the N-terminal extension that has little in common with the other two E2s. Ube2E2 interacts with ARA54 and RNF8. The RNF8/ Ube2E2 interaction is found to catalyze K48-linked poly-ubiquitination. Ube2E2 may play a role in ubiquitination and subsequent proteasomal degradation of the E3 Ub ligase Parkin.

Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified6xHis-Ube2E2 (UbcH8)