商品编号


A2201



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Proteasomes / Activators / Subunits



Size

100 ?g




商品信息

PA28 (also called the 11S regulatory complex) is another activator of the 20S proteasome, which assembles on either one or both ends of the 20S proteasome in an ATP-independent manner. PA28 can greatly enhance the peptidase activities of the 20S proteasome, but not for degradation of ubiquitinated proteins. The PA28 activator is a complex of two alternating homologous subunits, PA28α and PA28β, which assembles as a hexameric ring with an α3β3 stoichiometry. PA28α and PA28β are induced by interferon. Functionally, PA28 enhances the generation of class I peptides for antigen presentation. The third subunit, PA28γ, was recently identified in the nucleus. Recombinant PA28α and PA28β form a heptomeric ring; whereas PA28γ forms a hexameric ring. All of them can stimulate the peptidase activities of the 20S proteasome.

Additional Information






Product Name:

6xHis-PA28beta



Also Known As:

PSME2;PA28B; REGbeta; PA28beta;PA28-beta;PA28 beta



Catalog No.:

A2201



Size:

100 ?g



Molecular Weight:

27.4 kDa



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~95% by SDS-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

NM_002818



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s Lane 2: 5 ?g purified 6xHis-PA28beta













Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 ?M Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.






Shipping Method:

Dry ice shipping



References:

1. Ma CP,
et al
. (1992) J Biol Chem 267(15), 10515 – 10523.
2. Whitby FG,
et al
. (2000) Nature 408(6808), 115 – 120. 3. Cascio P,
et al
. (2002) The EMO Journal 21, 2636 – 2645.
4. Sijts EJAM,
et al
. (2011) Cell Mol Life Sci 68(9), 1491 – 1502.




Details

PA28 (also called the 11S regulatory complex) is another activator of the 20S proteasome, which assembles on either one or both ends of the 20S proteasome in an ATP-independent manner. PA28 can greatly enhance the peptidase activities of the 20S proteasome, but not for degradation of ubiquitinated proteins. The PA28 activator is a complex of two alternating homologous subunits, PA28α and PA28β, which assembles as a hexameric ring with an α3β3 stoichiometry. PA28α and PA28β are induced by interferon. Functionally, PA28 enhances the generation of class I peptides for antigen presentation. The third subunit, PA28γ, was recently identified in the nucleus. Recombinant PA28α and PA28β form a heptomeric ring; whereas PA28γ forms a hexameric ring. All of them can stimulate the peptidase activities of the 20S proteasome.

Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s Lane 2: 5 ?g purified 6xHis-PA28beta













Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 ?M Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.