商品编号


A1501



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Proteasomes / Activators / Subunits



Size

50 ?g










商品信息

Upon stimulation with IFN – γ, the expression of the three catalytic β subunits β1, β2, and β5 with iso-forms β1i (LMP2), β2i (MECL – 1), and β5i (LMP7) are induced, respectively. These subunits are incorporated into the 20S proteasome to form the immune 20S proteasome. It was reported that the immunoproteasome has altered proteolytic activities compared to its normal form, which favor the generation of immunopeptides for antigen presentation.

Additional Information






Product Name:

Bovine immuno 20S proteasome



Also Known As:

Immuno 20S proteasome



Catalog No.:

A1501



Size:

50 ?g



Molecular Weight:

700 kDa



Species:

Bovine



Source:

Bovine red blood cells



Stock:

20 mM Tris, 20 mM NaCl, 1 mM EDTA, 5 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~95% by native-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

N/A



Image(s):

(Click image to enlarge)










Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s Lane 2: 5 ?g purified Bovine immuno 20S proteasome


Coomassie-stained native-PAGE
Lane 1: 5 ?g purified Bovine immuno 20S proteasome


















Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28α (Cat. # A2100), the proteasome activity was assayed by using 50 ?M Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 ?M Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28γ (Cat. # A2300), the proteasome activity was assayed by using 50 ?M Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.






Shipping Method:

Dry ice shipping



References:

1. Goldberg AL,
et al
. (1992) Nature 357(6377), 375 – 379.
2. Aki M,
et al
. (1994) J Biochem 115(2), 257 – 269. 3. Tanaka K (1994) J Leukoc Biol 56(5), 571 – 575.
4. Zaiss DM,
et al
. (2011) J Immunol 187(5), 2302 – 2309.




Details

Upon stimulation with IFN – γ, the expression of the three catalytic β subunits β1, β2, and β5 with iso-forms β1i (LMP2), β2i (MECL – 1), and β5i (LMP7) are induced, respectively. These subunits are incorporated into the 20S proteasome to form the immune 20S proteasome. It was reported that the immunoproteasome has altered proteolytic activities compared to its normal form, which favor the generation of immunopeptides for antigen presentation.

Images:
(Click image to enlarge)










Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s Lane 2: 5 ?g purified Bovine immuno 20S proteasome


Coomassie-stained native-PAGE
Lane 1: 5 ?g purified Bovine immuno 20S proteasome


















Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28α (Cat. # A2100), the proteasome activity was assayed by using 50 ?M Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 ?M Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28γ (Cat. # A2300), the proteasome activity was assayed by using 50 ?M Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.