商品编号


A1401



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Proteasomes / Activators / Subunits



Size

50 ?g










商品信息

The 20S proteasome has a barrel – shaped structure arranged as four heptomeric rings of αββα. In eukaryotes, each of α and β ring is composed of seven different proteins. The β1, β2 and β5 subunits have ‘caspase-like’, ‘trypsin-like’ and ‘chymotypsin-like’ activities, respectively. In 26S proteasome-mediated protein degradation, to entry the β chamber of the 20S proteasome that houses the proteolytic sites, a substrate protein has to pass through a substrate translocation channel consisting of the double-ring formed by six ATPases of PA700 and the α chamber formed by α subunits of the 20S proteasome.

Additional Information






Product Name:

Bovine 20S proteasome



Also Known As:

20S proteasome



Catalog No.:

A1401



Size:

50 ?g



Molecular Weight:

700 kDa



Species:

Bovine



Source:

Bovine red blood cells



Stock:

20 mM Tris, 20 mM NaCl, 1 mM EDTA, 5 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~95% by native-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

N/A



Image(s):











Coomassie-stained native-PAGE
Lane 1: 5 ?g purified Bovine 20S proteasome

Coomassie-stained native-PAGE
Lane 1: 5 ?g purified Bovine 20S proteasome


















Activation of 5 nM 20S proteasome by 25 nM PA700 (Cat. # A1300), the proteasome activity was assayed by using 50 ?M Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Activation of 5 nM 20S proteasome by 25 nM PA28α (Cat. # A2100), the proteasome activity was assayed by using 50 ?M Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 ?M Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.






Shipping Method:

Dry ice shipping



References:

1. Waxman L,
et al
. (1987) J Biol Chem 262(6), 2451 – 2457.
2. Ganoth D,
et al
. (1988) J Biol Chem 263(25), 12412 – 12419. 3. Coux O,
et al
. (1996) Annu Rev Biochem 65, 801 – 847.
4. Kim HM,
et al
. (2011) Biochimica Biophysica Acta 1809(2), 67 – 79.




Details

The 20S proteasome has a barrel – shaped structure arranged as four heptomeric rings of αββα. In eukaryotes, each of α and β ring is composed of seven different proteins. The β1, β2 and β5 subunits have ‘caspase-like’, ‘trypsin-like’ and ‘chymotypsin-like’ activities, respectively. In 26S proteasome-mediated protein degradation, to entry the β chamber of the 20S proteasome that houses the proteolytic sites, a substrate protein has to pass through a substrate translocation channel consisting of the double-ring formed by six ATPases of PA700 and the α chamber formed by α subunits of the 20S proteasome.

Images:










Coomassie-stained native-PAGE
Lane 1: 5 ?g purified Bovine 20S proteasome

Coomassie-stained native-PAGE
Lane 1: 5 ?g purified Bovine 20S proteasome














Activation of 5 nM 20S proteasome by 25 nM PA700 (Cat. # A1300), the proteasome activity was assayed by using 50 ?M Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Activation of 5 nM 20S proteasome by 25 nM PA28α (Cat. # A2100), the proteasome activity was assayed by using 50 ?M Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 ?M Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.