商品编号


40611



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Kinase(s)



Size

20 ?g

商品信息





Species: Human

Host Species: Sf9 insect cells

MW: 69.5 kDa





Genbank #: AF245447

Tag(s): N-terminal His-tag

a.a: full length





Description:
Human Sphingosine kinase 2 (GenBank Accession No. AF245447), full length with N-terminal His-tag, MW=69.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.




Synonym(s):
SphK2, sphingosine kinase 2, SPK2




Specific Activity:
20 U/?g




Unit Definition:
A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.




Assay Conditions:
Sphingosine kinase activity was determined using D-erythro-sphingosine and ATP as substrates. Assay condition: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl
2
, 1 mM DTT, 3 ?M Na orthovanadate, 0.5 mM ATP, 4 ?M D-erythrosphingosine, and 0.75 μg/ml sphingosine kinase 2 at 37°C for 30 min. ATP reduction is detected using Kinase-Glo
?
Luminescent Kinase Assay Platform (
Promega
Corporation, M
ADI
son, WI).




Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 20% glycerol, and 3 mM DTT.




Format:
Aqueous buffer solution




Storage / St
ABI
lity:
>6 months at –80°C




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):

1. Zemann, B., et al., Blood.107(4):1454-8 (2006)
2. Maceyka, M., et al., J Biol Chem. 280(44):37118-29 (2005)

Application Reference:

1.
Synthesis and
BIOLOG
ical evaluation of carbocyclic analogues of pachastrissamine
(2015)
2.
Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation
(2014)




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Kinase/Lipids




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.