商品编号


40610



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Kinase(s)



Size

20 ?g

商品信息





Species: Human

Host Species: Sf9 insect cells

MW: 46.9 kDa





Genbank #: NM_001142601.1

Tag(s): N-terminal His-tag

a.a: full length





Description:
Human Sphingosine kinase 1 or SphK1 (GenBank Accession No. AF200328), full length with N-terminal His-tag, MW=46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system.




UniProt
Q9NYA1-1




Synonym(s):
SphK1, sphingosine kinase 1, SPK1




Specific Activity:
3,300 pmole/min/μg




Unit Definition:
A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.




Assay Conditions:
Assay was done in Kinase buffer containing 20 μM Sphingosine and 20 μM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Assay was done in Kinase buffer (40 mM Tris, pH 7.4, 20 mM MgCl2+, 0.1 mg/ml BSA) containing 20 μM Sphingosine and 20 μM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (
Promega
)




Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 225 mM imidazole, and 3 mM DTT.




Format:
Aqueous buffer solution




Storage / St
ABI
lity:
>6 months at –80°C.




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):

1. Delon, C., et al., J. Biol. Chem 279 (43) 44763-44774 (2004).
2. Taha, TA., et al., FASEB J. 20(3):482-4 (2005).

Application Reference:

Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation
(2014)




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Kinase/Lipids




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.