商品编号


40641



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Kinase(s)



Size

20 ?g

商品信息





Species: Human

Host Species: Sf9 insect cells

MW: 209 kDa (complex)





Genbank #: U79143 (p110α), XM_043865 (p85α)

Tag(s): N-terminal FLAG-tag(p110α), no tag on p85α

a.a: full length





Description:
Complex of N-terminal FLAG-tagged recombinant full-length human p110α (GenBank Accession No. U79143), with H1047R mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system. p110α(H1047R) MW=125 kDa, p85α MW=84 kDa.




Synonym(s):
PIK3CA, p110alpha, Phosphoinositide-3-Kinase, Catalytic, Alpha, PIK3R1, Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha), GRB1, p85alpha




Specific Activity:
1204 pmol/min/μg.




Assay Conditions:
25 μl kinase reaction is conducted in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl
2
, 100 μM ATP, 200 μM of PI3K substrate (BPS Cat.# 40560) and the PI3K enzyme for 15 minutes at 30°C. Conversion of ATP to ADP is detected using ADP-Glo? Luminescent Kinase Assay reagents (
Promega
Corp., M
ADI
son, WI).




Formulation:
25 mM Tris-HCl, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.03% Tween-20, 3 mM DTT, 50% glycerol.




Format:
Aqueous buffer solution




Storage / St
ABI
lity:
>6 months at –80°C




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):
1. Engelman, J.A., et al. Nature Med. 14:1351-1356 (2008)
2. Hafner, C., et al., Proc. Natl. Acad. Sci. USA. 104(33):13450-13454 (2007)
3. Serra, V., et al., Cancer Res. 68(19):8022-8030 (2008)




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Kinase/Lipids




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.