商品编号


40625



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Kinase(s)



Size

20 ?g

商品信息





Species: Human

Host Species: Sf9 insect cells

MW: 130 kDa





Genbank #: NM_002649

Tag(s): N-terminal His-tag

a.a: full length





Description:
Human p120γ (gamma) (GenBank Accession No. NM_002649), full length with N-terminal His-tag, MW=130 kDa, expressed in a Baculovirus infected Sf9 cell expression system.




UniProt
P48736




Synonym(s):
PI3CG, 3-kinase catalytic subunit gamma isoform; p120-PI3K; PI3K-gamma, p120γ




Specific Activity:
1200 pmol/min/μg.




Assay Conditions:
A 25 μl kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.4), 20 mM MgCl
2
, 100 uM ATP, 200 μM PI:PS substrate (BPS catalog #40560) and 0-200 ng p120γ enzyme for 15 minutes at 30°C. ATP reduction is detected using ADP-Glo? Kinase Assay Platform (
Promega
Corporation, M
ADI
son, WI).




Formulation:
25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 3 mM DTT, and 50% glycerol




Format:
Aqueous buffer solution




Storage / St
ABI
lity:
>6 months at –80°C




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):

1. Webb, LM., et al., J Immunol. 175(5):2783-7 (2005).
2. Voigt, P., et al., J Biol Chem. 280(6):5121-7(2005).

Application Reference:

Discovery of a potent and isoform-selective targeted covalent inhibitor of the lipid kinase PI3Kα
(2013)




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Kinase/Lipids




Product Type:
Recombinant Protein




Data shown is lot-specific.
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for specific information on other lots.