商品编号


40628



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Kinase(s)



Size

20 ?g

商品信息





Species: Human

Host Species: Sf9 insect cells

MW: 230 kDa (complex)





Genbank #: NM_005026.2 (p110δ), XM_043865 (p85α)

Tag(s): N-terminal GST-tag (p110δ), no tag on p85α

a.a: full length





Description:
Complex of N-terminal GST-tagged recombinant full-length human p110δ (GenBank Accession No. NM_005026), and recombinant full length, human p85α, GenBank Accession No. XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system. p110δ MW=146 kDa, p85α MW=84 kDa.




Synonym(s):
PIK3CD, p110delta, Phosphoinositide-3-Kinase, Catalytic, Delta, PIK3R1, Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha), GRB1, p85alpha




Specific Activity:
520 pmol/min/μg.




Unit Definition:
A unit of p110δ/p85α kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3,4,5-Trisphosphate/min at 30°C.




Assay Conditions:
A 25 μl kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.4), 20 mM MgCl
2
, 100 uM ATP, 200 μM PI:PS substrate (BPS catalog #40560) and p110δ/p85α enzyme for 15 minutes at 30°C. ATP reduction is detected using ADP-Glo? Kinase Assay Platform (
Promega
Corporation, M
ADI
son, WI).




Formulation:
25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.03% Tween-20, 3 mM DTT, 50% glycerol.




Format:
Aqueous buffer solution




Storage / St
ABI
lity:
>6 months at –80°C




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):
1. Vanhaesebroeck et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94:4330.
2. Vanhaesebroeck et al. (1999) EMBO J. 18:1292.




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Kinase/Lipids




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.