商品编号


50275



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Metabolic Enzymes



Size

200 ?g

商品信息





Species: Human

Host Species:
E. coli


MW: 42 kDa





Genbank #: NM_000154

Tag(s): N-terminal His-tag

a.a: 2 – 399 (end)





Description:
Human galactokinase (GenBank Accession No. NM_000154), full length with N-terminal His-tag, MW=42 kDa, expressed in an
E. coli
expression system.




UniProt
P51570




Synonym(s):
GALK1




Specific Activity:
≥176 pmoles/min/?g




Assay Conditions:
A 50 μl Galactokinase reaction is conducted in a buffer containing 20 mM Tris (pH 7.4), 5 mM MgCl
2
, 50 mM NaCl, 50 μM ATP, 1 mM DTT and 1 mM substrate D-Galactose at room temperature for 1h. ATP reduction is detected using Kinase-Glo? Luminescent Kinase Assay Platform.




Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 225 mM imidazole, and 3 mM DTT.




Format:
Aqueous buffer solution




Storage / St
ABI
lity:

>6 months at –80°C




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):
1. Thoden J.B. et al., J. Biol. Chem. 280 (10), 9662-9670 (2005).
2. Holden H.M. et al., Cell. Mol. Life Sci. 61 (19-20), 2471-2484 (2004).




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Metabolic Enzymes-Kinase




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.