商品编号


71077



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Metabolic Enzymes



Size

10 ?g

商品信息





Species: Human

Host Species: Sf9 insect cells

MW: 118.6 kDa





Genbank #: NM_145059

Tag(s): N-terminal FLAG-tag

a.a: 2–1084(end)





Description:
Human fucokinase (GenBank Accession No. NM_145059), a.a. 2-1084(end) with N-terminal FLAG-tag, MW = 118.6 kDa, expressed in baculovirus using an Sf9 insect cell expression system.




UniProt
Q8N0W3




Synonym(s):
fucokinase, FuK




Specific Activity:
≥85.3 pmole/min/?g




Assay Conditions:

The reaction mixture contained 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 2 mM L-Fucose, 100 μM ATP, and various amount of fucokinase in a final volume of 50 μL. The reaction was incubated at 37°C for 1 hr, and the amount of ATP consumed was calculated using Kinase-Glo Plus
?
reagent (
Promega
).




Formulation:
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 20% glycerol, and 80 ng/?l FLAG peptide




Format:
Aqueous buffer solution




Storage / St
ABI
lity:

>6 months at –80°C




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):
1. Hinderlich, S., et al., Biochem Biophys Res Commun. 2002 Jun 14; 294(3):650-4.
2. Zeitler, R., et al. J. Enz. Inhib. 1997 Feb; 11(4):265-73




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Metabolic Enzymes-Kinase




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.