商品编号


40617



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Kinase(s)



Size

10 ?g

商品信息





Species: Human

Host Species: Sf9 insect cells

MW: 60 kDa





Genbank #: NM_022766

Tag(s): C-terminal FLAG-tag

a.a: 1–537(end)





Description:
Human ceramide kinase (CERK), GenBank Accession No. NM_022766, a.a. 1-537(end) with C-terminal FLAG-tag, MW= 60 kDa, expressed in a baculovirus expression system.




UniProt
Q8TCT0




Synonym(s):
CERK, Ceramide Kinase, Lipid kinase 4, LK4, Acylsphingosine Kinase




Specific Activity:
≥90 pmole/min/?g




Unit Definition:
A unit of ceramide kinase activity is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C.




Assay Conditions:
CERK activity was measured at 37°C for 25 minutes in a buffer (20 mM HEPES pH 7.2, 80 mM KCl, 3 mM CaCl
2
, 10 mM MgCl
2
, 2 mM DTT, 5 μM ATP) using a beta-Octylglucoside mixed micellar assay with 100 μM Ceramide (BPS# 40618). ATP reduction was detected using Kinase-Glo
?
Luminescent Kinase Assay Platform (
Promega
, M
ADI
son, WI).




Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% Glycerol and 100 μg/mL Flag peptide.




Format:
Aqueous buffer solution




Storage / St
ABI
lity:
>6 months at –80°C.




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):
1. Mitra,P. et al., FEBS Lett. 581 (4), 735-740 (2007)
2. Kim,T.J. et al., Biochem. Biophys. Res. Commun. 342 (2), 611-617 (2006)




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Kinase/Lipids




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.