商品编号


40002



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Kinase(s)



Size

10 ?g

商品信息





Species: Human

Host Species: Sf9 insect cells

MW: 66 kDa





Genbank #: NM_004217

Tag(s): N-terminal GST-tag, C-terminal His-tag

a.a: 2–344





Description:
Human Aurora kinase B (GenBank Accession No. NM_004217), a.a. 2-344, with N-terminal GST-tag and C-terminal His-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.




Synonym(s):
Aurora Kinase B, STK12, STK5, AIM-1, IPL1, ARK2, AURB




Specific Activity:
6.2 U/?g.




Unit Definition:
One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per minute at pH 7.4 and 30°C.




Assay Conditions:
Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 4 uM aurora B substrate, and 4 ug/ml Aurora B kinase.




Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione




Format:
Aqueous buffer solution




Storage / St
ABI
lity:

>6 months at -80°C.




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):
1. Delaval, B. et al., Oncogene 23 (26), 4516-4522 (2004).
2. Yasui, Y. et al., J. Biol. Chem. 279, 12997-13003 (2004).




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Kinase/Serine-Threonine




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.