商品编号


40004



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Kinase(s)



Size

10 ?g

商品信息





Species: Human

Host Species: Sf9 insect cells

MW: 50 kDa





Genbank #: NM_003600

Tag(s): N-terminal His-tag

a.a: 2–403





Description:
Human Aurora kinase A (GenBank Accession No. NM_003600), a.a. 2-403 with N-terminal His-tag, MW=50 kDa, expressed in a Baculovirus infected Sf9 cell expression system.




Synonym(s):
Aurora Kinase A, STK15, STK6, BTAK, HARK1, ARK1, AURA




Specific Activity:
45 pmol/min/?g




Unit Definition:
One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per minute at pH 7.4 and 30°C.




Assay Conditions:
50 mM HEPES, pH 7.4, 3 mM MgCl
2
, 3 mM MnCl
2
, 1 mM DTT, 3 ?M Na orthovanadate, 0.5 mM ATP, 4 ?M Aurora A substrate, and 4 ?g/ml Aurora A kinase.




Formulation:
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.




Format:
Aqueous buffer solution




Storage / St
ABI
lity:

>6 months at -80°C.




Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.




Reference(s):
1. Ouchi,M. et al., J. Biol Chem. 279 (19), 19643-19648 (2004).
2. Kunitoku, N. et al., Dev. Cell 5 (6), 853-864 (2003).




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Kinase/Serine-Threonine




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.