商品编号


THRV3-100



品牌


MCLab



公司


MCLAB.Inc



公司分类


Protease



Concentration

2 mg/ml


Size

500 units (0.5 mg)

商品信息


MCLAB
's HRC3C Protease is fused with both GST and His tags, so that the protease is easily removed form cleavage reactions by either immobilized glutathione (GSH) resin or immobilized Ni-chelating resin. Turbo HRV3C Protease is highly active at 4?C, and also active in a variety of commonly used buffers, providing more flexibility in experimental design to keep the activity and intact structure of the target proteins.




Description:

MCLAB
's HRC3C Protease is fused with both GST and His tags, so that the protease is easily removed form cleavage reactions by either immobilized glutathione (GSH) resin or immobilized Ni-chelating resin. Turbo HRV3C Protease is highly active at 4?C, and also active in a variety of commonly used buffers, providing more flexibility in experimental design to keep the activity and intact structure of the target proteins.
Application:

MCLAB
's Turbo Human Rhinovirus (HRV) 3C Protease is a recombinant cysteine protease used to remove fusion tags from proteins with the HRV3C cleavage sequence. HRC3C Protease is highly specific for the PreScission Site, Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro, and cleaves between the Gln and Gly residues.
Supplied With:
HRV 3C Substrate, 100 ?l
10x HRV3C Protease Reaction buffer
HRV3C Protease 10X Cleaveage Buffer:
50 mM Tris-HCl (pH 8.0)
150 mM NaCl
Figure:
Figure 1. SDS-PAGE Analysis of Control Protein by Turbo HRV3C Protease
A 70 kD GST-fusion protein (1) at 1 mg/ml is reacted with Turbo HRV3C Protease at a ratio of 1:100 (w/w) in cleavage buffer at 4?C for 4 hour (2) 3 days (3) The cleaved products are 45 kD and 25 kD.
Source:
E. coli
Supplied In:
50 mM Tris-HCl (pH 8.0)
150 mM NaCl
1 mM EDTA
1 mM DTT
20% glycerol
Unit definition:
1 unit will cleave >95% of 100 ?g His-tag fusion control protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4°C for 16 h.
Recommended Storage Condition:
-20°C