商品编号


AG-40T-0545-C025



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

25 ?g

商品信息


More Information



Product Details



Synonyms

Probable Ubiquitin C-terminal Hydrolase FAF-X; Deubiquitinating
eEnzyme
FAF-X; Fat Facets in Mammals; FAM; Ubiquitin Thioesterase FAF-X; Ubiquitin-specific-processing Protease FAF-X



Product Type

Protein



Properties



Source/Host

Sf21 cells



Sequence

Human
USP
9x Isoform 2 (Accession Nr. Q93008) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In HEPES, NaCl, DTT and Glycerol.



Other Product Data


Use:
Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.05-0.5?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin Specific Peptidase 9, X-linked (
USP
9x), also known as DFFRX, FAF and FAM, is a cytoplasmic protein that belongs to the peptidase C19 family of deubiquitinating enzymes. It is 2570 amino acids (aa) in length with a predicted molecular weight of 292.3 kDa. Human
USP
9x shares 98% aa sequence identity with the mouse and rat orthologs.
USP
9x contains a catalytic domain located between aa 1531 and 1971 and a ubiquitin-like domain that is located at aa 873-963. It is widely expressed and has been shown to interact with and deubiquitinate multiple substrates. Some putative targets include β-Catenin, TOR, synuclein-α, the ubiquitin ligases (E3s) Itch, MARCH7, and SMURF1 and the MAP kinase kinase kinase ASK1.
USP
9x has also been shown to control the activity of the AMPK-related kinases ARK5 and MARK4 through direct removal of Lys29- and Lys33-linked ubiquitin chains. It has also been shown to promote cell survival by deubiquitinating and st
ABI
lizing the anti-apoptotic protein Mcl-1, and to regulate chromosome alignment and segregation during mitosis by deubiquitinating the chromosome passenger protein survivin. Additionally,
USP
9x appears to promote TGF-β signaling via deubiquitination of mono-ubiquitinated Smad4.
USP
9x expression has been shown to be correlated with several cancer types.