商品编号


AG-40T-0544-C025



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

25 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin C-terminal Hydrolase 8; Deubiquitinating Enzyme 8; Ubiquitin Isopeptidase Y; Ubiquitin Thioesterase 8; Ubiquitin-specific-processing Protease 8; UBPY



Product Type

Protein



Properties



Source/Host

Sf9 cells



Sequence

Human
USP
8 (Accession Nr. P40818) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In HEPES, NaCl, DTT and Glycerol.



Other Product Data


Use:
Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 10-50nM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin Specific Peptidase 8 (
USP
8), also known as ubiquitin isopeptidase Y (UBPY), is a widely expressed deubiquitinating enzyme belonging to the peptidase C19 family. It has a predicted molecular weight of 127.5 kDa. Human
USP
8 is 1118 amino acids (aa) in length and shares 84% aa sequence identity with the mouse and rat orthologs. It contains a N-terminal MIT domain (aa 33-116) that mediates endosomal localization, CHMP-binding, and maintenance of ESCRT-0.
USP
8 also has a rhodanese domain (aa 181-319) that binds NRDP1 ubiquitin ligase (E3), a SH3 domain binding sequence (aa 405-413), and a C-terminal catalytic domain (aa 734-1110).
USP
8 is a growth-regulated enzyme that controls the internalization and endocytic trafficking of cell surface receptors. Some receptors are targeted for internalization and degradation by ubiquitination.
USP
8 has been shown to disrupt the down-regulation of multiple receptors, including EGF R/ErbB1, ErbB2/Her2 and Smoothened, via their deubiquitination. Conversely,
USP
8 appears to have the opposite effect on the trafficking of CXCR4, PAR2, and the δ-opioid receptor. Depletion or catalytic inactivation of
USP
8 st
ABI
lized their expression. It is thought that deubiquitination of these receptors down-stream of the sorting endosome commits them to lysosomal degradation.
USP
8 can be phosphorylated at Ser680 allowing for 14-3-3-epsilon binding, which subsequently inhibits
USP
8 activity. Additionally,
USP
8 undergoes tyrosine phosphorylation at its N-terminus following EGF activation of the EGFR/ErbB1/ErbB2/Her2 receptor complex.