商品编号


AG-40T-0563-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g


商品信息


More Information



Product Details



Synonyms

Ubiquitin-like Carboxyl-terminal Hydrolase 18; ISG15-specific Protease; Ubl thioesterase 18



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Recombinant mouse
USP
18 (aa47-368) (Accession Nr. Q9WTV6) fused to N-terminal His-tag.



Crossreactivity

Mouse



MW

~38kDa



Purity

≥98% (SDS-PAGE)



Formulation

Liquid. In 50mM HEPES, pH7.5, 100mM NaCl, 10% glycerol and 1mM TCEP.



Other Product Data


Use:
Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant mouse
USP
18 concentration of 100-250nM when using ISG15-AMC (AG-40T-0274MC) as a substrate.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





USP
18 plays an important role in antiviral responses and interferon-mediated signaling pathways. Mice lacking the gene are hypersensitive to interferon, have shortened
Lifespan
s and pathologic abnormalities of the brain. While it was initially speculated that the cause of the defects resulted from the accumulation of ISG15-conjugated intracellular proteins (a target of
USP
18 catalytic function), it was later demonstrated that
USP
18 has multiple signaling roles, at least one of which is independent of its ISGylase activity.