商品编号


AG-40T-0537-C025



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

25 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin C-terminal Hydrolase 14; Deubiquitinating Enzyme 14; Ubiquitin Thioesterase 14; Ubiquitin-specific-processing Protease 14; TGT



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human
USP
14 (Accession Nr. P54578).



Crossreactivity

Human



Formulation

Liquid. In HEPES, NaCl, DTT and EDTA.



Other Product Data


Use:
Ubiquitin-specific deconjugating enzyme. The activity was measured at 2.0 RFU/min/?g using an enzyme concentration of 18?M and a Ub-AMC concentration of 4?M in 100mM HEPES, pH 8.5, 10mM DTT, and 5% Glycerol at 25 °C.
USP
14 activity is greatly stimulated in the presence of 19S proteasome.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin Specific Peptidase 14 (
USP
14) is a cytoplasmic protein that belongs to the peptidase C19 family of deubiquitinating enzymes. It has a predicted molecular weight of 56.1 kDa. Human
USP
14 is 494 amino acids (aa) in length, contains a N-terminal ubiquitin-like (Ubl) domain (4-80 aa), and shares 97% aa sequence identity with the mouse and rat orthologs.
USP
14 is one of three deubiquitinating enzymes associated with the 26S Proteasome. It revers
IBL
y binds the 19S regulatory particle via its Ubl domain and mediates the disassembly of ubiquitin chains on substrates by the stepwise removal of ubiquitin molecules from the distal tip of the chain.
USP
14 has also been shown to deubiquitinate the chemokine receptor CXCR4, regulating both CXCL12/SDF-1-induced chemotaxis and receptor degradation.
USP
14 appears to be critical for the development and function of neuromuscular junctions. Additionally,
USP
14 is thought to serve as a physiological inhibitor of endoplasmic reticulum-associated degradation under non-stressed conditions by inhibiting the degradation of unfolded endoplasmic reticulum proteins.