商品编号


AG-40T-0535-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin C-terminal Hydrolase 1; Deubiquitinating Enzyme 1; Ubiquitin Thioesterase 1; Ubiquitin-specific-processing Protease 1



Product Type

Protein



Properties



Source/Host

Sf21 cells



Sequence

Human
USP
1 (Accession Nr. O94782 ) fused to a C-terminal His-tag, and glycine-to-alanine substitutions at positions 670 and 671.



Crossreactivity

Human



Formulation

Liquid. In HEPES, NaCl, Glycerol and TCEP.



Other Product Data


Use:
Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.01-0.5?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





USP
1 (Ubiquitin-Specific-processing Protease 1), also known as ubiquitin carboxyl-terminal hydrolase 1, is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the fanconi anemia pathway. Reported substrates of
USP
1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA.
USP
1 plays important roles in DNA damage responses and cancer-related processes and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy. By itself,
USP
1 is nearly completely inactive and requires a protein binding partner, UAF1 (
USP
1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity. In addition to the naturally occurring substates listed above, the
USP
1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains.