商品编号


AG-40T-0533-C025



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

25 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-like Modifier-activating Enzyme 5; THIFP1; UBE1DC1; UBA5



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UFM1 Activating Enzyme (Accession Nr. Q9GZZ9) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 7.5, 100mM NaCl, 1mM TCEP.



Other Product Data


Use:
Member of the UFM1-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a UFM1-conjugating (E2) enzyme and a UFM1 ligase (E3) to conjugate UFM1 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 50-200nM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-fold Modifier 1 (UFM1) Activating Enzyme, also known as ubiquitin-like modifier activating enzyme 5 (UBA5) and ubiquitin-activating enzyme E1 domain-containing protein 1 (UBE1DC1), is a 404 amino acid (aa) member of the ubiquitin-activating (E1) enzyme family with a predicted molecular weight of 45 kDa. The mouse and rat UFM1 Activating Enzyme/UBA5 orthologs share 87% and 88% aa sequence identity with the human protein, respectively. UFM1 Activating Enzyme/UBA5 is highly conserved in plants and metazoans but not in yeast. In humans, it is expressed both as the full length protein and as a truncated isoform that lacks aa 1-56. It is widely expressed in human cells where it is localized to the cytoplasm and nucleus. UFM1 Activating Enzyme/UBA5 has a conserved ATP-binding motif in close proximity to an active site cysteine residue, Cys250 in humans, and a metal binding motif, both of which are common to most E1 enzymes. It is the activating enzyme for both UFM1 and SUMO2. UFM1 Activating Enzyme/UBA5 is required for erythroid differentiation in mice.