商品编号


AG-40T-0524-C025



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

25 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin C-terminal Hydrolase L1; PARK5; PGP9.5; Neuron Cytoplasmic Protein 9.5; Ubiquitin Thioesterase L1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UCH-L1 (Accession Nr. P09936).



Crossreactivity

Human



Formulation

Liquid.



Other Product Data


Use:
Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial enzyme concentration of 10-50nM. 15 minute pre-incubation with 10mM DTT is recommended to achieve maximum activity.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin Carboxyl-terminal Esterase L1 (UCH-L1), also known as PGP 9.5, is a deubiquitinating enzyme with a predicted molecular weight of 25 kDa. The human protein shares 95% amino acid sequence identity with its mouse and rat orthologs. UCH-L1 is expressed abundantly in neurons, accounting for 1-2% of total soluble proteins in the brain. It localizes primarily to the cytoplasm, but a subpopulation has been shown to be transiently nuclear. UCH-L1 contains two catalytic residues, Cys90 and His161, which are required for isopeptide bond cleavage at the C-terminal glycine residue of ubiquitin. The levels of free ubiquitin appear to be partially regulated by UCH-L1 through the hydrolysis of small ubiquitin chains and the st
ABI
lization of monomeric ubiquitin. Mice lacking functional UCH-L1 show neuronal dysfunction and neurodegeneration, and mutations in this enzyme have been linked to Parkinson's disease, suggesting that it is important for proper central nervous system function. UCH-L1 also likely plays a complex role in cancer. It has been reported to function as an oncogene in lymphoma, colorectal cancer, and nonsmall cell lung carcinoma. In contrast, it is thought to function as a tumor suppressor protein in nasopharyngeal and breast cancers.